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Leachate Generated from a Public Abattoir in Nigeria is a Repository of Escherichia coli Carrying a High Burden of Extended Spectrum Β-Lactamase (ESBL) Genes

Journal: Acta Microbiologica Bulgarica (Vol.38, No. 2)

Publication Date:

Authors : ;

Page : 98-106

Keywords : Abattoir leachate; uidA; ESBL- producing Escherichia coli; antibiotic resistance; multidrug resistance; blaSHV; blaCTX-M; blaTEM.;

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Indiscriminate dumping of wastes from abattoirs has contributed to the proliferation of antibiotic resistance in the environment. Most studies in Nigeria have focused on the phenotypic detection of extended spectrum β-lactamase (ESBL) production in bacteria from the abattoir environment, with few carrying out ESBL genotyping of the isolates. This study characterized and genotyped ESBL- producing Escherichia coli associated with leachate generated from the waste dumps of a public abattoir. Presumptive ESBL-producing E. coli were isolated on chromogenic medium from leachates collected from three designated points. Fifty-five non-duplicated isolates showing carriage of uidA gene were selected for further studies. The resistance manifested to a panel of five selected antibiotics by the fifty-five isolates was: imipenem (0%), enrofloxacin (14.5%), sulfamethoxazole-trimethoprim (69.1%), oxytetracycline (78.2%) and cefotaxime (98.2%). Thirty-five of the isolates showed resistance to three or more classes of antibiotics and were termed multidrug-resistant. It was observed that blaCTX-M was the most predominant ESBL gene with 51/55 (92.7%) isolates carrying the gene, 27/55 (49.5%) carried blaTEM while one isolate carried blaSHV. Twenty-four isolates (43.6%) carried two of the three targeted ESBL genes, while one isolate carried all the three ESBL genes. Twenty-eight of the isolates (50.9%) carried only one gene, while two isolates were devoid of any of the ESBL genes. This study has shown that abattoir leachates is a repository of multidrug resistant and ESBL- producing E. coli, highlighting the need for adequate waste disposal and proper waste treatment in abattoirs.

Last modified: 2022-07-06 14:46:54