Collagen Matrices from Leather Industry Wastes for Biomedical Application

Modern biomedical science is challenged to develop new wound healing drugs. The collagen-containing wastes of leather industry could be the rich source of collagen products for further use in biomedical science. The aim of this research was to find the best source of collagen between limed pelt, delimed pelt and fleshings of cattle hides, and to prepare it for the use as a matrix for further microbiological studies. Collagen was extracted with 0.5 M acetic acid and 5 mM EDTA. The purity of the extracted collagen was checked by gel-electophoresis (SDS-PAGE). The rate of growth and crystal violet assay of laboratory strains (S. aureus, P. aeruginosa) were used for microbiological evaluation of obtained collagen matrices. The delimed pelt provided the highest concentration of collagen and the greatest volume of collagen products. All obtained collagen products were applicable as matrices for microbial cells growth. The applicability of collagen products from leather industry wastes for biomedical studies in Ukraine was shown.