Mathematical Analysis of Drug-Drug Combinations under Non-Constant Ratio on the Cutaneous T-Cell Lymphoma Cell Line Hut-78

A set of designed experiments for evaluation of the combination effects between the alkylphospho¬choline erufosine (ERF) and micellar curcumin with prolonged release (MCRM) combined in non-con¬stant ratio were performed on the cutaneous T-cell lymphoma cell line HuT-78. Each non-constant ratio between ERF and MCRM doses consisted of five different concentrations of ERF (2, 4, 8, 16 and 32 μM) combined with one of three rationally chosen concentrations of MCRM (3.75, 7.5 or 15 μM). For the first time, a program with non-linear medium effect-dose model was coded in the MAPLE® software and used in all calculations. As a final calculation of the step by step methodology, the combination index (CI) was obtained to quantitatively determine the synergistic or additive effects of the designed combinations. The best synergism was achieved when using the lowest concentration of MCRM – 3.75 μM.