Study of Hypoglycemic Properties of Fresh Leaves of Bryophyllum pinnatum Extracted in Aqueous Medium by Using Ultrasound

Bryophyllum pinnatum is a medicinal plant widely used for several purposes in different tribes in Bangladesh. From ethnobotanical study it was observed that several ethnic groups used these leaves for the treatment of diabetes. The present study was designed to scrutinize the anti-diabetic properties of the leaves of B. pinnatum. The study was also emphasized on developing a green extraction method. In this study ultrasound assisted extraction in aqueous medium from fresh plant's parts was applied and compared with two conventional methods such as aqueous decoction and the ethanol cold extraction method. Glucocorticoid hormone dependent diabetes was created by the intraperitoneal injections administration of the dexamethasone on the Swiss albino mice used as diabetic animal models. Hypoglycemic potency of the crude extracts of B. pinnatum leaves was compared to the standard antidiabetic drug glibenclamide. However, healthy control and diabetic control groups were also used to minimize the biasness. After two weeks of treatment, a significant change was observed in hypoglycemic status in the plasma. The effects of crude extracts on body weight was also examined, however, no significant correlation was observed.