In Vitro Seed Germination, Seedlings and SPSs Development in a Terrestrial Medicinal Orchid Eulophia Graminea Lindl. of Bangladesh

Full strength MS medium with PGRs combination gave the topmost response (86.67%) of seeds germination descended by PGRs supplemented PM (80.00%), VW (53.34%) and KC (46.67%) media. The lowest required time for initiation of seed germination was recorded on full strength PGRs fortified MS medium (10.37 ± 0.26 wks) followed by PM (12.37 ± 0.28 wks), VW (14.07 ± 0.42 wks) and KC (17.63 ± 0.35 wks) media. Half strength PGRs free KC, MS, PM and VW media did not show any response. The minimum time required for development of protocorms, differentiation of first leaf primordia, differentiation of first root primordia and development of seedlings was recorded on full strength PGRs fortified MS medium (14.57 ± 0.31 wks), (19.23 ± 0.30 wks), (25.60 ± 0.35 wks) and (32.77 ± 0.35 wks) accordingly. The liquid MS medium containing 2.0 mg/l BAP + 1.2 mg/l NAA showed best results in terms of SPSs development (94.67% response and 6.24 ± 0.14 wks time). Well-developed rooted seedlings were transferred to environment by successive phases of acclimatization.