Development and Validation of LCMS Method for the Estimation of Pramipexole in Human Plasma

Background: An accurate and precise LCMS method was developed for the determination of pramipexlole in human plasma. Method: Separation of the drug was achieved on Hypersil gold column using a mobile phase consisting of Acetonitrile : Ammonium Formate (65:35). Quetiapine Fumarate was used as an internal standard. Solid Phase extraction technique was used for pramipexole using Strata-X 33 μm cartridges. Samples were ionized by positive-ion electro spray ionization mode. The mass transitions m/z 212.00 to 153.00 and 384.700 to 220.300 were used to measure Pramipexole and Quetiapine Fumarate respectively. The assay was calibrated over the range 20 to 4000 pg/ml with correlation coefficient of 0.9928. Results: Validation data showed were within the limits. No matrix effect was found in different sources of human plasma tested. Mean extraction recovery of pramipexole was satisfactory.