Study of the extraction process of the Pleurotus citrinopileatus mushroom and evaluation of the biological activity of the extract

Pleurotus citrinopileatus, also known in Vietnam as golden oyster mushroom, is a food rich in protein, fiber, essential amino acids, carbohydrates, water-soluble vitamins, and minerals. In particular, in the composition of yellow oyster mushrooms, there is β -1.3 - glucan with high biological activity as an active ingredient contributing to the intense stimulation of immunity. The study researched the process of extracting Pleurotus citrinopileatus mushroom by the assisted extraction of a combination of ultrasonic waves and pyrolysis in an aqueous solvent at 100°C, then evaluation of the biological activity of the extract to orient the application of functional foods to support cancer treatment. Some of the conditions of the Pleurotus citrinopileatus extraction process documented through the study are as follows: The solvent used for extraction was water, sodium chloride salt was used as a catalyst, with a ratio of 1g NaCl:100 ml of solution, the sample was ultrasonically processed for 60 min and magnetic stirred at 100°C for 2 hours, the concentration of Na2CO3 participating in the reaction was 20%, HCl concentration was 2M, the ratio of IPA treatment/mushroom residue was 1:1 (v/v). From Pleurotus citrinopileatus mushroom powder, the research team successfully obtained β- glucan.