Iron Chelation, Hydroxyl and DPPH Radical Scavenging Efficacies of Cobalt (II)- 2- Guanidinobenzimidazole Complex |Biomedgrid

The guanidine and imidazole derived moieties have gained significant attention in nucleophilic organocatalysis, anion recognition, coordination and pharmaceutical chemistry. The present study was designed to understand the biochemical efficacy of the Cobalt (II)- 2- Guanidinobenzimidazole [Co (II)-2GBI] complex. For the purpose, we measured the Fe (II) chelation efficacy of the stated complex. Co (II)-2GBI exhibited significantly (p< 0.005) higher potential at 10, 20 & 40 uM as compared with the standard, EDTA. Infact, 95% chelation was recorded at 50 ug/mL. One of the products of Fenton reaction is hydroxyl radical. Significant hydroxyl radical scavenging potential was noted. Infact 61% activity was the highest recorded at 50μg/mL. It was further confirmed by DPPH scavenging potential, where the complex inhibited 85 % (DPPH) at 50 μg/mL. The IC50 values of the complex are 25.01, 29 and 40.63μg/mL against, Fe (II) chelation, DPPH and hydroxyl quenching assay. The presence of nitrogen containing moieties in complex may contribute towards the broad antioxidant potential. However, further molecular studies are needed to decode the exact biochemical mechanism of action.