Development and Validation of UV Spectroscopic Method for Estimation of Lamivudine in Tablet Dosage Form

It is simple, rapid sensitive precise and specific UV spectrophotometric method for the determination of lamivudine in bulk drug and tablet dosage form were developed and validated. A simple double beam UV spectroptometric method has been developed and validated with different parameter such as linearity, precision .The drug freely soluble in DMF. The drug was identified in term of solubility studies and on the basis of melting point done on melting point apparatus of equiptronics. It showed absorption maxima were determined in DMF. The drug obeyed the beer's law and sowed a good correlation of concentration with absorption which reflex in linearity .The UV spectroscopic method was developed for estimation of lamivudine in tablet dosage form and also validated as per ICH guidelines. The drug is freely in DMF slightly soluble in methanol and practically insoluble in acetone so the DMF is used as a diluentin the method. The melting point of lamivudine was found to be 160- 1610c . It showed absorption maxima 268 nm is an DMF . On the basis of the absorption spectrum, the working concentration was set on 10µg/ml (PPM). The linearity was observed between 10-50 µg/ml. The result of the analysis were validated by recovery studies. The recovery was found to be 99.2% for three level respectively.