Effect of Laser Activated Sodium Hypochlorite on Sealing Ability of Two Endodontic Sealers Using Stereomicroscope-An In vitro Study

Introduction: The combination of various irrigants, irrigation techniques along with root canal sealers helps in achieving a three-dimensional seal in root canals, which is of clinical relevance. The present in-vitro study aims to assess and compare the efficacy of laser-activated NaOCl on the sealing ability of two endodontic sealers. Materials and Methods: Forty extracted human single-rooted permanent teeth were collected and decoronated 12 mm from their apices. Instrumentation did with an 11 mm working length using Rotary Pro taper files to F3. All the teeth grouped into four (n= 10) based on irrigation protocol and sealer used as follows: Group A1 – Laser activated NaOCl + AH Plus Group A2 – Laser activated NaOCl +nano seal, Group B1 – NaOCl + AH Plus, Group B2 – NaOCl + nano seal. The lateral condensation technique did obturation. Roots stored in freshly prepared 1% methylene blue for 72 hours. The roots were split longitudinally along the long axis using a diamond disc underwater coolant, and markings made at 2, 4, and 6 mm from the apex. The depth of dye penetration and microleakage examined under a stereomicroscope (Magnus) at 30× magnification. Results: Statistical analysis carried out by using the software, statistical package for social sciences [SPSS]. Results represented in the form of minimum, mean, and standard deviation. Conclusion: The laser-activated NaOCl group exhibited better sealing ability than the control group. Among the two sealers, the Bio root RCS sealer showed less leakage & better sealing ability than AH plus Clinical Significance: Increasing the temperature of low concentration sodium hypochlorite solution by the action of the diode laser, not only enhances its immediate tissue dissolution capacity but also increased the dentin permeability by removing the smear layer. Further leading to better adhesion between the root canal sealer and the gutta-percha with the radicular dentin.