The Alteration of Gut Microbiota Between Patients with Left Ventricular Ejection Fraction Preserved and Reduced Heart Failure |Biomedgrid

Aims: In this study, 16S rRNA sequencing was used to reveal the changes of the composition and the metabolic pathways of intestinal flora in patients with Heart Failure (HF). Methods and results: Twenty patients with HF and five healthy adults were included. Patients were divided into group O (healthy adults), group P (LVEF ≥50%,), and group M (LVEF < 50%). Stool samples were collected on admission. High-throughput sequencing combined with bioinformatics analysis was used to detect the diversity of 16S rRNA gene V4 region of bacteria in the three groups. OTU dilution curves of all samples showed a gentle trend, indicating that the sequencing depth was appropriate. Compared with healthy adults, the composition of intestinal microflora in patients with HF was significantly different, mainly showing increased abundance of microbacili, Lactobacillus, Lactobacillus, Staphylococci and Staphylococci. The abundance of Roseburia, Megamonas and Catenibacterium increased in the HF group with LVEF ≥50% compared with LVEF < 50%. Decreases occurred in Clostridiales, Veillonella, Sutterella, and Bilophila. Preliminary functional analysis of the flora showed that genes involved in Trimethylamine-n-Oxide (TMAO) synthesis, lip-polysaccharide synthesis, and lipid metabolism pathways were significantly increased in the HF group with LVEF ≥50% compared with the healthy adult group. At the same time, there was no significant difference between LVEF ≥50% and <50% group. Compared with healthy adults, the predicted phenotypes in the HF group were mainly an increase in gram-positive bacteria, a decrease in aerobic bacteria and in gram-negative bacteria. Conclusions: Compared with healthy adults, the gut microbiota of patients with HF is significantly unbalanced. The functional changes of intestinal flora in HF were preliminarily elucidated by bioinformatics functional metabolic analysis.