Effects of substrate stiffness and anti-cancer drug on morphology and viability of Prostate cancer cell lines

Cancer cell detection in various tissues of the body, as well as during chemical medication treatment, can aid in identifying these cells and providing faster and more effective treatments. Studies have been conducted to aid in the diagnosis and identification of cancer cells. Although significant progress has been made, there is still a need for research in this sector, particularly when multiple variables are considered at the same time. The present study has the novelty of examining the simultaneous effects of cell culture substrates and chemical drugs on the morphology of two cancer cell lines with different degrees of invasion. In the current study, two cell lines with varying degrees of metastasis were cultivated on three surfaces with varying elastic modulus, and their morphology was studied using photography and image processing with code and software. In this study, the cell morphology was evaluated with great precision. In addition, the cell viability of these two cell lines was assessed for the aforementioned groups in order to determine the rate of cell death in each group. The findings show that drugs have a greater impact on cell morphology than substrates and cell types, and that the two cell lines respond differently to drug and substrate combinations, with DU145 cells being more susceptible to drug-induced cytotoxic effects. primarily on soft substrates. Understanding these interactions is critical for adapting treatments to the specific properties of prostate cancer cells, which could improve outcomes for patients with various metastatic tendencies.