Electrolyte state of canine red blood cells during hypothermic storage with the addition of N-acetylcysteine

Aim. To evaluate the effect of adding N-acetylcysteine (NAC) to SAGM (adenine-glucose-mannitol saline) solution on the electrolyte composition and pH of canine erythrocytes during hypothermic storage. Material and Methods. Red blood cells were stored in SAGM solution with or without NAC at 4–5 C. The concentrations of sodium, potassium, chloride, and pH were determined on days 0, 7, 21, and 35 of storage. Results. The sodium concentration increased in both groups, slightly less in the experimental group with NAC. The accumulation of potassium was less pronounced in the NAC-supplemented group. Chloride levels remained stable, and pH decreased, particularly in the experimental group. Conclusions. NAC contributes to the stabilization of the electrolyte environment during storage, in particular to potassium retention and pH control.