CHEMICAL I N VESTIGATION AND EVALUATION OF ANTIMICROBIAL ACTIVITY OF TRICHODESMA EHRENBERGII S CHWEINF. EX BOISS.GROWING WIDELY IN GEBEL ELBA, EGYPT

In this study, the primary phytochemical and biological screeningof the aerial parts of Trichodesma ehernbergii Schweinf. e x Boiss. (Family; Boraginaceae) has been done. Phytochemical analysis showed the presence of phen ols, flavonoids, alkaloids, terpenoids, steroids, tannins, saponins, and sugars in different extracts of the aerial partsof T. ehernbergii . The concentration of phenols, flavonoids, alkaloids and tannins and saponinswere foundin the range 138 ±0.8 mg/g (as g allic acid equivalent), 123 ±0.6 mg/g (as rutin equivalent), 1.3 ±0.6 mg/g, 2.7 ±0.2 mg/g and 6.7 ±0.2 mg/g, respectively. Combined and free amino acidsanalysis showed the presence of 17 amino acids asfree and protein amino acids. Glutamic acid (29.919ppm) and lysi ne (72.640ppm) represented as the major components of free and protein amino acids, respectively. The antimicrobial activity ofmethanolextractof the aerial parts of T. ehernbergii against 6 bacterial strains and 5 fungal strainsby a pour plate technique metho d was determined. Keywords : Trichodesma ehernbergii , Gebel Elba, Phytochemical screening, Primary and Secondary metabolites, Eco - physiological analysis, Amino acids, Antimicrobial Activity.