EVALUATION OF ANTI-DIABETIC ACTIVITY OF CAESALPINIA SAPPAN WOOD AGAINST ALLOXAN INDUCED DIABETIC RATS

The Caesalpinia sappan L. (Fabaceae) was authenticated and identified. The aim of this study is to evaluate the anti-diabetic activity of ethanolic extract of Caesalpinia sappan wood in albino wistar rat models (in-vivo) and inhibition of α-glucosidase enzyme method (in-vitro). The ethanol extract dose of 100mg/kg and 200mg/kg was significantly reduced blood glucose levels in diabetic rats after 21 days were evaluated for anti-diabetic activity in alloxan induced diabetic rats. The results were determined by blood parameters and the histopathological study showed significant anti diabetic activity. Invitro method of anti-diabetic activity, the ethanolic extract was prepared 25 ?g/ml to 1000?g/ml. The invitro study shown better inhibition of α-glucosidase enzyme was determined by % inhibition. The ethanolic extract of Caesalpinia sappan showed IC50 is 215.95±7.52 and standard drug showed 183.46±5.85. Caesalpinia sappan shows that alkaloids and flavanoids present in this extract may be possibly responsible for the antidiabeic activities.