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Journal: Fisheries Science of Ukraine (Vol.32, No. 2)

Publication Date:

Authors : ; ; ;

Page : 51-60

Keywords : Key words: silver carp; bighead carp; age-1; age-1+; age-2; genetic-biochemical markers; genetic structure; alleles; genotype; heterozygosity.;

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Purpose. Studying the peculiarities of the genetic structure of different age groups of silver and bighead carps from fish farm Limanske with the use of genetic-biochemical markers. Methodology. The methods of vertical polyacrylamide and horizontal starch electrophoresis with our own modifications have been used for the study. Sampling of the biological material and histochemical staining of gel plates were carried out using the generally accepted methods. Statistical analysis of the obtained data were performed in "Biosys-I". Findings. We analyzed the genetic structure of age-1, age-1+ and age-2 of silver and bighead carps with the use of genetic-biochemical markers - Рralb, EST, MDH, МЕ, СА. We demonstrated the peculiarities of allele frequencies and genotypic composition of protein system loci. A high level of heterozygosity in the examined was detected loci for age-1 silver carp ? 66.7-88.9% and age-1 bighead carp ? 65.4-77.8%. A significantly higher level of the observed average heterozygosity compared to the expected heterozygosity of age-1 as silver carp (Ho = 75.9%, He = 49,6%), and bighead carp (Ho = 73,6%, He = 47,9%) was observed. This fact indicated on the necessity for performing a stabilization of their genetic structure. The calculated Wright's F index in different age groups showed a predominance of the observed level of the average heterozygosity over the expected one for 22.7?53% in silver carp and 24.5-53.7% in bighead carp. A stabilization of the genetic structure in age-2 silver carp (Ho = 57.8%; He = 47.1%) by the heterogeneity level was observed. Originality. For the first time we analyzed the genetic structure and the level of heterogeneity of the breeding groups of age-1, age-1+, age-2 silver and bighead carps from fish farm Limanske with the use of genetic-biochemical markers. Practical value. The results of the study can be used in selection and breeding works aimed at creating the breeding stocks of silver and bighead carps to control the level of interspecies genetic variability. The obtained experimental data of the genetic structure analysis of silver and bighead carps of different age groups will be used for the monitoring of the genetic structure changes during their adaptation to new environmental conditions.

Last modified: 2015-06-23 15:56:05