Detection and identification of Streptococcus pyrogenes es from ENT patients by different methods

Streptococcus pyogenes (S. pyogenes ) is a Gram-positive bacterium tainting the upper respiratory tract, such as the tonsils and pharynx, and it also induces post-infection diseases such as rheumatic fever and glomerulonephritis. The study aim was to compare between different methods for detection and identification of S. pyogenes from Ear, nose and throat (ENT) patients. A total of 10 (ten) S. pyogenes strains were analyzed, these were recovered from throat cultures of 150 patients with (ENT). Identification of bacteria carry out depending on the conventional biochemical tests, Strepto System 9R, using universal PCR primer, followed by RFLP, and finally amplification of the specific genes which included spy 1258 and dnase B. The molecular methods shows, that all S. pyogenes isolates co