Evaluation Of antibacterial activity Of methanol and acetone extracts (different parts) of moringa oleifera and hibiscus rosasinensis

Methanol and acetone extract of two plant genus; Moringa oleifera and Hibiscus rosa-sinensis was carried out to identify the particular part of plant having antibacterial activities and also analyze phytochemicals constituents. Crude extracts were tested for antibacterial activity with concentrations of 200 and 300μg/ml. Acetone extracts of Moringa oleifera seed (MOS) and Moringa oleifera fruit pulp (MOFP) showed significant antibacterial activity against test organisms; for S.aureus, P.aeroginosa at 20 μg/ml and for E.coli, and S.typhi at 300μg/ml. While, methanol extracts of MOS and MOFP also shown activity against test organism except against S.typhi at 300μg/ml. Meanwhile, among Hibiscus rosa-sinensis leaf (HRL), Hibiscus rosa-sinensis flower petal (HRFP) and Hibiscus rosa-sinensis flower clayx (HRFC), only HRFC of acetone and methanol extracts shown significant activity at 300 μg/ml against S.aureus, E.coli and P.aeroginosa but no activity was shown against S.typhi. Surprisingly, methanol and acetone extracts of leaf extracts from both the plants did not show any activity on test organism. Subsequently, phytochemical analysis of acetone extracts supposed that compounds like, methionine, moringine, nitriles, isothiocynate, thiocarbonates and saponins might be involved in inhibiting the bacterial growth. Thus, seeds and pulp of M. oleifera & calyx of H. rosa-sinensis can be used as potential sources to extract antimicrobial agents possessing medicinal values.