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Development of a RP-HPLC Method for the Estimation of Metronidazole Benzoate Oral Suspension

Journal: Pharmaceutical and Biological Evaluations (Vol.1, No. 1)

Publication Date:

Authors : ;

Page : 41-43

Keywords : Species; Metronidazole benzoate oral suspension; antiamoebic; RP-HPLC; validation;

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A new reversed-phase high performance liquid chromatography method was validated for the determination of Metronidazole benzoate in suspension. The validation was achieved on Biosil column (250 ´ 4.6 mm) acetonitrile & potassium dihydrogen phosphate (pH 4.0) as mobile phase at a flow rate 1.2 ml/min. Detection was carried out using UV detector at 320 nm. The developed and validated method was successfully applied for the quantitative analysis of Metronidazole benzoate oral suspension. The total chromatographic analysis time per sample was 6 min at retention time of about 4.36 min. The standard curve was linear over the concentration ranges 1-10 mg/ml. The value obtained of Limit of detection (LOD) was 0.439 and Limit of quantitation (LOQ) was 0.197 mg/ml. The proposed method is fast accurate and precise for the determination of Metronidazole benzoate for routine quality control of suspension containing this drug.

Last modified: 2015-08-14 15:08:12