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Journal: Indo American Journal of Pharmaceutical Sciences (IAJPS) (Vol.2, No. 8)

Publication Date:

Authors : ; ; ; ; ;

Page : 1190-1197

Keywords : Biomolecules; DPPH assay; Intoxication; Hepatocyte; SGOT; SGPT; SALP.;

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The main aim and objective of the present research work was to extract the potential biomolecules present in the fruit seeds of Glycosmis pentaphylla L and to evaluate the in vitro and in vivo Hepatoprotective activity. The in vitro Hepatoprotective activity was carried out by DPPH assay. The IC50 value (50% inhibition) of the EEFSGP was found to be 212.96 ?g/ml. The in-vivo Hepatoprotective activity was carried out by using albino rats. The results displayed that the elevated levels of SGOT, SGPT, ALP and Serum bilirubin were mainly due to CCl4 intoxication, reduced significantly (*P<0.05) in rats, after treatment with ethanolic extract of fruit seeds of Glycosmis pentaphylla (EEFSGP). Treatment with EEFSGP at a dose of 250 mg/kg b.w. decreased the SGOT, SGPT, ALP, Serum Bilirubin levels by 6.23% ns(non significantly), 28.96, 8.81, and 11.11%ns (non significantly) respectively, while a higher dose of 500 mg/kg b. wt. was more effective, causing a reduction of 25.02, 47.65, 24.09, and 27.35%. Silymarin used as standard showed a reduction of 55.09, 68.98, 57.46 and 35.04% receiving CCl4 alone. So depending upon the experimental data it was confirmed that the biochemical parameters of the group treated with ethanolic extract was significantly lower than the CCl4 treated group. Moreover the treatment with the extract significantly reduced the previously raised levels of AST, ALT, ALP and bilirubin in hepatotoxic rats. Histopathological investigation displayed that at both doses (250 mg/kg b.w. and 500 mg/kg b.w.), the EEFSGP was possessed moderate to good hepatoprotective activity, but at 500 mg/kg b.w. executed excellent hepatoprotective activity against CCl4 induced damaged hepatocytes. Key words : Biomolecules, DPPH assay, Intoxication, Hepatocyte, SGOT, SGPT, SALP.

Last modified: 2015-08-24 02:20:54