STABILITY INDICATING UV-SPECTROPHOTOMETRIC METHOD FOR SECNIDAZOLE IN BULK AND PHARMACEUTICAL FORMULATION

Secnidazole is a nitroimidazole anti-infective agent which has chemical structure susceptible to degradation. It shows activity against anaerobic micro-organisms and appears effective in the treatment of amoebiasis, giardiasis, trichomoniasis and bacterial vaginosis. In this work forced degradation studies of secnidazole were carried out by developed & validated spectroscopic method. A simple, accurate, precise and economical spectroscopic method have been developed and validated for the determination of secnidazole in their bulk and pharmaceutical formulation at 320 λ max in double distilled water by using UV-spectrophotometer. Beer’s law was obeyed in the concentration range of 4-12 ppm. Good accuracy (90.50%), precision (%RSD 1.50), LOD (0.33μg/ml), LOQ (1.003μg/ml) and linearity (0.993) were obtained. The stress degradation study was carried out according to the ICH requirements (Q1A (R2) and Q1B) include acid, alkali, H2O2, and photolytic degradation. The result showed that the drug degrades more under alkaline (37.76%) and acidic (21.33%) condition than other stress conditions. The proposed method and stress degradation study can be used for routine quality control analysis of secnidazole in bulk and pharmaceutical formulation.