Ameliorative effect of Jasminum grandiflorum (L.) leaves against cyclophosphamide (CTX) induced renal and liver toxicity

In the present study, first we screened the ethanolic extract of Jasminum grandiflorum leaf (EEJG) through the in-vitro DPPH free radical scavenging method for its possible antioxidant activity and was found to posses good antioxidant potential. After that, ameliorative effect of the EEJG was evaluated against Cyclophosphamide (CTX) induced renal and hepatic toxicity. Wistar albino rats were divided into five groups, Group-I, control (0.5% w/v, Carboxy Methyl Cellulose); Group-II, negative control (CTX, 30 mg/kg, i.p, for 15 days);Group-III, (EEJG, 100 mg/kg, b.w., p.o., + CTX 30 mg/kg, b.w., i.p, for 15 days); Group-IV, (EEJG, 200 mg/kg, b.w., p.o. + CTX, 30 mg/kg, b.w., i.p, for 15 days) and Group-V, (received only EEJG, 200 mg/kg, b.w., p.o, for 15 days). Extract and CTX were given once daily for 15 days. Administration of CTX (30 mg/kg, b.w.,i.p.) for 15 days induced renal and liver damage as indicated by the serum marker enzymes of liver alanine aminotransferase (SGPT), aspartate aminotransferase (SGOT), alkaline phosphatase (ALP), bilirubin and biochemical parameters for kidney function such as serum creatinine, urea and uric acid. Equivalent to these changes CTX also alters the hematological parameters like RBC, total WBC count and hemoglobin level. Concurrent treatment with EEJG (100 and 200 mg/kg, b.w., p.o.) effectively ameliorate the toxicological changes induced by the CTX through scavenging of free radicals. The Jasminum grandiflorum has potential as adjuvant treatment option to CTX for circumventing the side-effects associated with its antineoplastic applications.