A STUDY ON THE MITOCHONDRIAL COI DNA SEQUENCE AND PHYLOGENETIC STATUS OF ANASTATUS BANGALORENSIS MANI & KURIAN AND ANASTATUS ACHERONTIAE NARAYANAN, SUBBA RAO & RAMACHANDRA RAO (HYMENOPTERA: EUPELMIDAE)

Anastatus (Hymenoptera: Eupelmidae) species are end parasitoids of a wide variety of insect eggs of Hemiptera, Lepidoptera and Orthoptera. It included the family Eupelmidae of Hymenoptera and they are an important egg parasitoid of several major insect pests. Here we report the partial sequence of cytochrome oxidase sub unit I gene (COI) of Anatatus bangalorensis (KU052674) and Anastatus acherontiae (KU052673) and its phylogenetic relationship. The COI gene sequence of A. bangalorensis and A. acherontiae are showed considerable variation with other related species. The mitochondrial COI DNA barcode developed in this study can be used for the accurate identification. The COI partial coding sequence of A. bangalorensis (KU052674) are showed 1.6% difference to A. acherontiae (KU052673), A. bangalorensis and A. acherontiae demonstrates the efficiency of the barcoding gene in discriminating global phylogeographical variants among the related species complex. A. bangalorensis and A. acherontiae are in a same clade on the phylogenetic tree.