In vivo neuroprotection study of two 1,2,4-triazole derivatives in Drosophila melanogaster Oregon K flies

This study presents the neuroprotective effect of two 1,2,4-triazole derivatives namely, 4-amino-3-(4-hydroxybenzyl)-1H-1,2,4-triazole-5(4H)-thione (C1) and 4-amino-3-(3- methoxybenzyl)-1H-1,2,4-triazole-5(4H)-thione (C2) assayed in vivo using Drosophila melanogaster (oregon K strain) as a model for neuroprotection studies. Oxidative stress was induced by paraquat, a neuro toxin. The antioxidant efficacy of the molecules was evaluated through the determination of malondialdehyde (MDA), glutathione (GSH), antioxidant enzymes like superoxide dismutase (SOD) and catalase (CAT) in the homogenate of the flies. The induced oxidative stress by paraquat exposure was evidenced by significant increase in the level of MDA and declining in the level of GSH. In case of C1 and C2 pretreated flies, a significant diminish in the level of MDA, re-establishment of GSH level and modulatory effect on antioxidant enzymes were seen. The compound C1 exhibited better level of protective ability against the stress compared to C2.