CYTIDINE DEAMINASE ACTIVITY MEASUREMENT USING GEMCITABINE AS A SUBSTRATE

Cytidine deaminase enzyme activity is a high individualized indicator for the immune response to infection. The purpose of the research was to evaluate the activity of cytidine deaminase using atypical nucleoside ? gemcitabine as a substrate. Enzyme activity was measured for 80 plasma samples to compare cytidine versus gemcitabine using standard volumes and microplates. So, cytidine deaminase activity against gemcitabine comparable activity against the standard substrate - cytidine. For samples with high absolute values of activity gemcitabine is even higher than cytidine.