L-ARGININE AND L-NAME EFFECTS ON FUNCTIONAL AND PHYSICOCHEMICAL PROPERTIES OF HEMOGLOBIN IN CONDITIONS OF EXPERIMENTAL CHRONIC ALCOHOL INTOXICATION

T has been revealed that different ligand forms of hemoglobin in the peripheral blood of rats are redistributed under the experimental chronic alcohol intoxication (ECAI), including a reduction of oxyhemoglobin and growth of methemoglobin against the back- ground of total hemoglobin reduction. Also it has been found that under ECAI the con- centration of alkaline resistant hemoglobin is rising, whereas the affinity of rat hemoglo- bin for oxygen is falling. The per os consumption of NOS substrate (L-arginine) under ECAI causes a significant decrease in methemoglobin content in the blood of rats. As for the consumption of NOS-unspecific inhibitor (L-NAME), it leads to an increase in the affinity of rat peripheral blood hemoglobin for oxygen both under ECAI and in control group. The consumption of L-arginine by rats of control group decreases the affinity of hemoglobin to oxygen similar to EСАI. The absorption spectra of RHb and HbFe 2+ NO of rat’s blood were characterized at Sore band and in the ultraviolet region. It was found that rate of nitration of hemoglobin in vitro under L-arginine consumption increa?ses comparing with ECAI. In the groups of animals consumed L-NAME nitration of hemo- globin was slowed in control and in the group with ECAI.