Respons Superovulasi Sapi Peranakan Ongole terhadap Penyuntikan Tunggal Follicle Stimulating Hormone ke dalam Ruang Epidural (SUPEROVULATION RESPONSES IN ONGOLE CATTLE CROSSBREED TREATED WITH A SINGLE EPIDURAL INJECTION OF FOLLICLE STIMULATING HORMONE)

Super-ovulation is conventionally performed by injection of FSH twice daily for four days. This treatment needs frequent attention by farm-personnel and relatively increases the possibility of failures due to mishandling and errors in administration of the treatment. This study was conducted to evaluate the responses of superovulation trough a single injection of FSH into epidural space in ongole cattle crossbreed. In Experiment 1, a combination of single dose injection of FSH was applied into epidural space plus intramuscular (epi+i.m group) compared to the group of intramuscular injection of FSH, which was treated twice daily for four days (intramuscular group), using equal total dose of FSH 400 mg. Superovulation response of epi+im group (n=4) was not significantly different compared with intramuscular group (n=4). In experiment 2, it compared two treatmentof FSH in different concentration(280 mg versus 160 mg) in a single dose applied into epidural space. Data of Epi+im group from experiment 1 was used as control. Group of 280 mg FSH (n=4) resulted total collection of ova/embryo and transferable embryos (9,00+2,65 and 3,33+2,52 respectively) was significantly different compared to the 160 mg group (n=4) (which were 2,00+1,26 and 0,00) P <0,05, although they werenot significantly different compared to the control (9,33+5,68 and 3,67+3,21). In conclusion, injection of a single dose of FSH at 280 mg into epidural space result in a comparable transferable embryo which similar to the conventional method that applied intramuscular injection of FSH twice daily for four days.