DETERMINATION OF SOME CHLORINATED POLLUTANTS IN FISH SAMPLES OF PATOKU LAGOON

In this study are presented concentrations of organochlorine pesticides and polychlorinated biphenyls (PCB), in ten fish samples of Patoku Lagoon. Fish samples were taken in November 2014. For isolation of organochlorine pesticides and PCBs from biota samples of Patoku Lagoon, ultrasonic bath extractions (assisted with Hexane/Dichlormethane 3/1 as extraction solvent), were used. 10 g fresh fish samples were taken for analyzes. Clean-up procedure was performed by using firstly silicagel with 45% sulphuric acid and finally an “open” florisil column for clean-up procedure. Analysis of organochlorinated pesticides and PCBs were realized in simultaneously in Rtx-5 capillary column (30m x 0.32mm x 0.25μm). HP 6890 Series II, gas chromatograph equipped with μECD detector was used for this study. The organochlorine pesticides detected were HCHs (a-, b-, γ- and d-isomers), and the DDT-related chemicals (o,p-DDE, p,p-DDE, p,p-DDD, p,p-DDT), hexachlorobenzene (HCB), Heptachlor, Heptachlorepoxide, Aldrine, Dieldrine, Endrine, Methoxychlor and Mirex. Seven PCB markers were analyzed for evaluation of PCB concentrations in fish samples. For all fish samples were found concentrations in higher levels for pesticide residues and their metabolites and volatile PCB markers. These data could be because use of pesticides in adjacent agricultural areas near Patoku Lagoon. Water currents, atmospheric impact, stability of pollutants could be factors that affect pollution in Patoku Lagoon.