DEVELOPMENT AND VALIDATION OF TLC DENSITOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF ASPIRIN AND TICLOPIDINE HYDROCHLORIDE IN TABLET DOSAGE FORM

Accurate, specific, precise and robust TLC densitometric method has been developed for simultaneous estimation of Aspirin (ASP) and Ticlopidine hydrochloride (TIC) in tablet dosage form. The chromatographic separation was performed on precoated silica gel TLC 60 F254 plates using n-Hexane: Ethyl acetate: Methanol (6.5:1.5:2 v/ v/v) as mobile phase. This system was found to give compact bands for Aspirin and Ticlopidine hydrochloride (RF values 0.52 ± 0.05and 0.75 ± 0.05 respectively). Densitometric analysis of Aspirin and Ticlopidine hydrochloride were performed at 239 nm. Regression analysis data for the calibration plots were indicative of good linear relationships between response and concentration over the range 1000-5000 ng/band for Aspirin and 500-2500 ng/band for Ticlopidine hydrochloride. The method was validated as per ICH guidelines for accuracy, precision, LOD, LOQ and robustness Keywords: Aspirin (ASP), Ticlopidine hydrochloride (TIC)