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Caspase Dependent Apoptosis is Only Inhibited on Γ Irradiation of Cells Conditioned by Repetitive Oxidative Stress

Journal: International Journal of Science and Research (IJSR) (Vol.2, No. 9)

Publication Date:

Authors : ; ; ;

Page : 12-18

Keywords : gamma rays; Cytochrome c; Caspases; Apoptosis inducing factor;

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V79C cells were derived from V79 cell line by through chronic oxidative stress that was found to be radio-resistant. These cells had demonstrated transformation?like stable changes and could be used as model system to study oxidant induced carcinogenesis. Our objective was to understand mechanism of radiation-resistance in these cells. Apoptotic cell death was inhibited in these cells as visualized microscopically by Hoechst staining and nucleosomal ladder formation in agarose gel. Release of cytochrome c in cytoplasm and Apoptosis Inducing Factor in the mitochondrial and nuclear fraction of cells were determined by Western blotting. The caspase 9 and caspase 3 activities in these cells were estimated from fluorimetric assays. These results revealed that the radiation resistance was due to inhibition of caspase-dependent apoptotic death pathways although Apoptosis Inducing Factor mediated pathway remained unaffected. These findings may aid in understanding the mechanism of radiation resistance in tumors arising from oxidative stress.

Last modified: 2013-10-01 22:32:37