COMPARATIVE ANALYSIS OF CONTRIBUTION OF POLYMORPHISM OF GENETIC MARKERS TO FORMATION OF DIFFERENT PATHOLOGIES (BY THE EXAMPLE OF HYPERTENSION AND VIRAL HEPATITIS C)

The aim of the study was to analyze the contribution of IL-10, PPARGC1A, BCAT1 gene polymorphisms to the development of cardiovascular diseases (i.e., hypertension), as well as the contribution of IL-28B gene polymorphism to the successful response to interferon therapy in viral hepatitis C type 1. Evaluation of the contribution of genetic markers makes it possible to predict the risk of development and features of the disease as well as to develop optimal therapeutic regimens. Materials and methods: For the evaluation of PPARGC1A, IL-10, BCAT1 gene polymorphisms the patients were divided into two groups: the experimental group (patients with grade 3 hypertension) and the control group (healthy volunteers without cardiovascular diseases). To analyze the effect of the genetic SNP markers rs12979860 and rs8000917 of IL-28B gene on the effectiveness of interferon therapy we selected two groups of patients infected with hepatitis C virus type 1: those with and without response to therapy. Research material: human DNA extracted from peripheral blood leukocytes. Methods: polymerase chain reaction, restriction analysis, sequencing, high-resolution melting curve analysis. Results: The role of allelic and genotypic variants of the PPARGC1A, IL-10, BCAT1 loci in the formation of grade 3 hypertension was demonstrated to be ambiguous. A significant contribution of the studied single nucleotide substitutions 39743165T>G (rs8099917) and 39738787C> T (rs12979860) of gene IL-28 B to the formation of a successful response to interferon therapy in viral hepatitis C type 1 (Fst= 13.51% and Fst= 8.63 %, respectively) was established.