The Effect of Serum from Acute Traumatic Brain or Spinal Cord Injury Patients on the Growth of Bone Marrow-Derived Mesenchymal Stem Cells (Atcc-USA)

Accelerated osteogenesis associated with traumatic brain injury BTI or spinal cord injury SCI is inconclusive and its cause remains obscured. The purpose of this study was to ensure a clinical evidence of its presence and to reveal the possible underlying mechanism. Healing indicators of diaphyseal femoral fractures in 20 patients with BTI and 20 patients with SCI were compared to 20 patients with femoral fracture only. The effect of sera of blood samples withdrawn from these patients on cell count proliferation rate of bone marrowderived Mesenchymal Stem Cells MSCs (ATCC-USA) were measured and compared to sera from 20 patients with BTI only, 20 patients with SCI only, and a control group. The results showed that femoral fractures with BTI or SCI heal more expectedly, faster with exuberant callus (p<0.001) and showed statistically significant increased cell count and growth rate of MSCs with sera from BTI and SCI patients with or without femoral fractures, 82.34%, 83.90%, 81.46%, and 81.50% respectively versus 52.96% in the control and 59.77% in patients with femoral fractures only (p<0.005). These results suggested enhancement of fracture-healing secondary to TBI and SCI due to the presence of factors in the serum that have a mitogenic effect on MSCs.