The mixed impact of chemical and physical factors on reproductive system of rats in conditions of cold stress

The results of experimental investigations on studying features of gonadotoxic effect of nitrobenzene (NB), methyl tertiary butyl ether (MTBE) and electromagnetic radiation (EMR) both in conditions of cold stress and in conditions of thermal acceptability are given. The investigations were carried out on laboratory animals, in 30-fold intragastric administration of NB or MTBE in a dose of 1/10 LD50 and exposition of animals in two different thermal regimens ? 4 hours a day, 5 times a week. Effect of EMR was simulated in 70 kHz frequency, 600 V/m voltage. The features of gonadotoxic effect of NB and MTBE were studied by the indices of spermatozoa functional state, general histological analysis of the state of testicle tissues, supplemented with morphometric values, was carried out,. The analysis of results of experimental research proves that morpho-functional changes of reproductive system in laboratory animals during the influence of chemical factors (NB and MTBE), physical factors (EMR) in two different thermal regimens are similar provided that there is the increase of gonadotoxic effect in mixed effect of mentioned factors and decreased temperature.