HPLC Calibration Process Parameters in Terms of System Suitability Test

During the routine analysis of drug and analytes System Suitability Test (SST) is one of the most important and integral parts of HPLC method development and calibration. System Suitability Test (SST) is generally performed to evaluate the suitability and effectiveness of the entire chromatographic system not only prior to use but also during the time of analysis. Due to several reasons the performance and the capacity of the entire chromatographic system may abruptly or mildly change during their regular uses. This modification of the system can in turn affect the reliability of the entire HPLC analytical results. For this reasons different parameters which is related to the operation of the whole chromatographic system can be monitored and investigated to find out the integrity and reliability of the whole HPLC systems. These parameters are used to determine characteristic chromatographic parameters, such as the number of effective theoretical plates, resolution, asymmetry, detection limit and selectivity. After the checking of the parameters, the system is then only can be declared suitable if the responses are within permitted limits. The objective of our present review article is to emphasize the importance of the System Suitability Test (SST) for development and validation of the HPLC analysis. In addition, we also discuss about the measurement and calculation of different SST parameters in very simpler way.