A Validated Method for the Determination of Firocoxib in Equine Tissues
Journal: Austin Chromatography (Vol.2, No. 3)Publication Date: 2015-05-26
Authors : Cox S; Hayes J; Gordon K; White M; Macpherson M;
Page : 1-4
Keywords : Firocoxib; HPLC; Tissue; UV detection;
Abstract
A new reversed-phase High Performance Liquid Chromatography (HPLC) method was developed and validated for the determination of firocoxib in equine tissue. Firocoxib was detected by ultraviolet detection at 290nm after undergoing a liquid extraction using ethyl acetate: hexane (40:60) and separation on a Sunfire C18 column. The mobile phase consisted of water with 0.025% trifluoroacetic acid and acetonitrile (50:50), with a flow rate 1.1 mL/min. A concentration range of 5-1500ng/gm produced a linear curve with r?>0.99. The lower limit of quantification was 5ng/gm. The intra and inter assay variability was <10% and the average recovery was 97%.
Other Latest Articles
- Development of High Performance Liquid Chromatography (HPLC) Method for the Estimation of Fosamprenavir Calcium in Pharmaceutical Formulation
- Comparison of the Detection Limit and the Quantification Limit of Nicotinamide on Different Chromatographic Sorbents in TLC
- Development of Liquid Chromatographic Method for Estimation of Ondansetron and Ranitidine in Combined Dosage Form
- The Ionization Technology of LC-MS, Advantages of APPI on Detection of PPCPs and Hormones
- Stability-Indicating HPTLC Method for Simultaneous Quantification of Moxonidine and Amlodipine Besylate in Their Combined Pharmaceutical Dosage Form
Last modified: 2017-02-15 19:18:46