ISOLATION OF PHYTO CONSTITUENTS FROM THE ROOTS OF COLEUS FORSKOHLII BY COLUMN AND FLASH CHROMATOGRAPHIC METHOD

The herb, Coleus Forskohlii Briq. belongs to family ‘labiatae' and is well known for its medicinal use throughout the plains of India. The roots of this plant are therapeutically active due to the presence of the diterpenoid, Forskolin. In the present research work, flash chromatographic method was developed for the isolation of three oleic acids from petroleum ether extract and 1-fluoroforskolin along with four other phyto constituents from methanolic extract of the roots of coleus forskohlii. Three compounds were isolated and identified from the petroleum ether extract of roots of C. forskohlii: 1. n-Hexadecanoic acid, 2. Cis-13- octadecenoic acid, 3. Cis-vaccinic acid and five compounds from methanolic extract of roots: 4. 1- Fluoroforskolin, 5.Stigmasterol, 6. Sclaral, 7. 2-dodecen-yl (-) succinic anhydride, 8. (3R, 4aS, 10aR)- Dodecahydro-3, 4a, 7, 7, 10a-pentamethyl-3-vinyl-1H-benzo[f] chromene. The characterization and confirmation of isolated compounds was done by GC-MS and 1HNMR spectral studies. Keywords: Coleus forskohlii, Labiatae, Flash chromatography, Forskolin, oleic acid.