POTENTIAL OF RESVERATROL PRODUCTION WITH BIOTRANSFORMATION BY YEAST USAGE

Resveratrol, a phytoalexin, excreted naturally by several plants such as grape, Japanese knotweed and peanut. Also, many of them contain some kinds of glycosides such as piceid which shows less bioactivity.
-Glucosidase is an enzyme that hydrolyzes
-glycosidic bonds between the reducing side of glucose and an aryl, alkyl aglycone or oligosaccharide. Therefore,
-glucosidase could be used for deglycosylation of piceid into resveratrol for a commercial application. In this research, hydrolysis of piceid in Polygonum cuspidatum to resveratrol by a patented microorganism Dekkera bruxellensis (BCRC 920084), secreting
-glucosidase was investigated. In addition,
-glucosidase for enhancing biotransformation efficiency was multiple used within whole cells under condition of liquid suspension. Then, a series of enlarging experiments were followed by 50-mL, 3-L and 10-L operation volume and the conversion rate was average 92.55 ± 9.66% after 5 times reused efficiency.