ISOLATION AND MOLECULAR IDENTIFICATION OF PESTICIDES TOLERANT BACTERIA IN AGRICULTURE SOIL OF RAMPUR (U.P)

Huge amounts of pesticides are applying every year in agriculture sector which help to increase the yield of crop which facilitate to fulfill the demand of food of increasing population. Pesticides controls harmful effects caused by the mark organisms. However, pesticides may unconsciously be transferred to the environment where they can impact non-target organisms. Micro organism in the soils developed capability to bioremediation of hazardous pesticides. In this work our aim is to identify pesticides tolerant bacteria in the agriculture soil. We collected the soil samples of vegetable filed. Isolation of pesticides tolerant bacteria were carried out using enrichment culture techniques containing pesticides as a sole source of carbon was isolated and selected for their ability to resistance against pesticides and antibiotics. We Identified 51 pesticides tolerant microorganisms in which 30 were showing Pseudomonas spps and remain were E.coli. Among 51, nine isolates were showing high pesticides tolerant.