Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for the proteomic based identification of Aggregatibacter actinomycetemcomitans isolated from orodental infections
Journal: International Archives of Integrated Medicine (IAIM) (Vol.4, No. 4)Publication Date: 2017-04-16
Authors : Ramanath Karicheri; Beena Antony;
Page : 8-13
Keywords : A.actinomycetemcomitans; Orodental infections; MALDITOF MS.;
Abstract
Aim: Aggregatibacter actinomycetemcomitans is a fastidious gram negative cocco bacilli responsible for aggressive and chronic periodontitis as well as other systemic infections. In this study the usefulness of MALDITOF MS a proteomic based study was evaluated for the identification of A. actinomycetemcomitans isolates from orodental infections. Materials and methods: Fifty clinical isolates of A. actinomycetemcomitans obtained from orodental infections were subjected for identification with conventional as well as MALDITOF MS analysis. Results: All the isolates tested were accurately identified by the MALDITOF MS analysis. Among the 50 isolates 35 were identified at secure and probable species level and another 15 were identified at highly probable species level. Conclusion: MALDITOF MS is reliable, cost effective and rapid test for the identification of slow growing fastidious bacteria like A. actinomycetemcomitans compared with conventional methods.
Other Latest Articles
- Age wise prevalence of developmental coordination disorder in school going children in west India
- Sarcomatoid Renal Cell Carcinoma: A Rare Case Report
- Incidental renal cell carcinoma with endometrial adenocarcinoma: A rare case report
- Evaluation of gastric carcinomas with high resolution transabdominal sonography
- Comparison of rectal, infra red tympanic and infra red skin temperature in term neonates
Last modified: 2017-04-27 18:36:21