Study of Seasonable Variation in Vasicine Content and Influence of Plant Growth Regulator on Callus Culture of Adhatoda vasica Nees

Adhatoda vasica is a well-known plant drug in Ayurvedic and Unani medicine. Adhatoda leaves have been used extensively in Ayurvedic Medicine primarily for respiratory disorders. Vasicine is the major alkaloid, responsible for pharmacological action. Vasicine was studies for seasonal variation and different concentrations of auxin and cytokinin was used to study their effect on callus formation. HPLC method was used to determine vasicine content.