Development of Conditions of Submerged Biosynthesis of Landomycin A

Background. Development of the process and biotechnology of production of antitumor antibiotic with microbial origin – landomycin A. Objective. Establishment of the rational parameters for the submerged biosynthesis of landomycin A during the cultivation in the laboratory conditions. Methods. Cultivation of the producer Streptomyces cyanogenus S136 was performed on shaking devices in the flasks. Antibiotic was extracted from culture fluid with ethyl acetate; the content of antibiotic was determined by measuring optical density of the extract. Results. Conditions and parameters for antibiotic submerged biosynthesis of the landomycin A by S. cyanogenus S136 were investigated. Alternative nutrient mediums for the biosynthesis of the landomycin A were presented. Mediums that are based on soybean's flour extract and corn's flour allow obtaining up to 80 mg/ml of antibiotic. The influence of mixing intensity and temperature on the rate of the product accumulation and its dynamics was established. Rational technological parameters of the producer's submerged biosynthesis such as temperature 25 ± 1 °С mixing intensity 230–250 U/min, duration of the submerged fermentation 50–60 hours were established. Conclusions. Variants of the culture media, that were selected (based on the corn's flour and the extract of the soybean's flour) and the parameters of the landomycin A biosynthesis, that were set are the basis for optimization and design of the technology for obtaining the antibiotic in industrial conditions.