DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF AGOMELATINE IN NANOSTRUCTURED LIPID CARRIERS
Journal: Indian Drugs (Vol.54, No. 3)Publication Date: 2017-03-25
Authors : Verma S. D.; Prajapati J. B.; Patel A. A.;
Page : 52-57
Keywords : ;
Abstract
The objective of work was to develop and validate a UV spectrophotometric method for determination of agomelatine (AGM) loaded in nanostructured lipid carriers (NLCs). The solvent and wavelength of detection were optimized in order to maximize sensitivity of proposed method. The method was validated for different parameters like linearity, precision, specificity, accuracy, limit of detection (LOD), limit of quantitation (LOQ) and robustness as per ICH guidelines. A wavelength maximum of AGM in methanol: chloroform (7:3V/V) mixture was found at 235nm. The method was found to be linear in the range of 1 to 6μg/mL with a correlation coefficient (r2) of 0.997. The accuracy of the method was studied by recovery study and % recovery was found in range of 99 to 100.11%. The LOD and LOQ were found to be 0.043μg/mL and 0.141μg/mL respectively. The method is simple, accurate and requires relatively inexpensive instrument. The method was used successfully for determination of AGM loaded into NLCs.
Other Latest Articles
- VALIDATED RP-HPLC AND UV-SPECTROSCOPY METHODS FOR THE ESTIMATION OF DAPAGLIFLOZIN IN BULK AND IN TABLETS
- EFFECT OF HYDROALCOHOLIC EXTRACT OF DRIED FRUITS OF TRAPA NATANS L ON ANIMAL MODELS OF COGNITIVE DYSFUNCTION
- FORMULATION AND EVALUATION OF MICROSPHERE BASED ORO DISPERSIBLE TABLETS OF SUMATRIPTAN SUCCINATE
- FORMULATION AND EVALUATION OF GASTRORETENTIVE FLOATING TABLETS OF CANDESARTAN CELEXETIL BY DIRECT COMPRESSION
- DETERMINATION OF ANTI-BACTERIAL ACTIVITY AND IN VITRO CYTOTOXIC ACTIVITY USING BRINE SHRIMP LETHALITY ASSAY OF CAESALPINIA BONDUCELLA LINN EXTRACTS
Last modified: 2017-07-20 17:51:21