Vitamin D supplementation attenuated carageenan-induced paw edema and formalin-induced nociception in indomethacin-treated rats

Background: This work evaluated the effect of vitamin D supplementation on carrageenan-induced paw edema and formalin-induced nociception in indomethacin treated rats. Methods: Rats were grouped into six (A ? F) of 5 each. Groups (C ? F) were pretreated for 14 days with vitamin D; 250, 500, 1000 and 1000 iu/day respectively. On day 15, they were treated as follows: group A (10 ml/kg distilled water), B; (10 mg/kg indomethacin), C ? E; (10 mg/kg indomethacin) F (1000 iu/day vitamin D alone). One hour later 50μl 1% carrageenan or 25μl 5% formalin was injected into the sub-plantal surface of left hind paw. For carageenan-induced paw edema, paw sizes were measured 1 h later, then every hour for 5h. For formalin-induced nociception, number of paw licking/lifting was counted for each rat from 0 ? 5 min (first phase) and 25 ? 30 min (second phase) post-formalin injection.