LEVELS OF BIOGENIC AMINES AND RELATED COMPOUNDS IN CEREBROSPINAL FLUID AND BLOOD PLASMA OF PATIENTS WITH NON-TRAUMATIC INTRACEREBRAL HEMORRHAGE

Characteristics of biogenic amines metabolism in non-traumatic intracerebral hemorrhage (ICH) remain virtually uninvestigated. The aim of study was to analyze the formation of biogenic amines and related compounds pool in cerebrospinal fluid (CSF) of ICH patients and to evaluate the ratio of its components in CSF and blood plasma. Material and methods. 105 ICH patients and 19 patients with cerebral infarc-tion (comparison group) were examined. CSF and blood samples were once obtained during the first three days after stroke onset and assayed byion pair high performance liquid chromatography for levels of dopamine precursors (tyrosine, Tyr;3,4-dihydroxyphenylalanine, DOPA) and metabolites (3,4 dihydroxyphenylacetic acid, DOPAC; 3-methoxy- 4hydroxyphenylacetic (homovanilic) acid, HVA). Levelsofserotonin(5-HT), its precursors (L-tryptophan, Trp; 5-hydroxy-L-tryptophan,5-HTP) and metabolite 5-hydroxyindolacetic acid (5-HIAA) were also determined. Results. CSF levels of Tyr and HVA in ICH were significantly higher versus comparison group while DOPA and DOPAC levels did not significantly differ. CSF concentration of 5-HT was significantly lower but Trp, 5-HTP и 5-HIAA – significantly higherversus comparison group. Blood plasma ICH patients levels of Tyr, DOPA and HVA were significantly higher versus comparison group while concentrations of 5-HT, Trp, 5-HTP and 5-HIAA did not significantly differ. Conclusions. There is the increase of dopamine and serotonin precursors and metabolites levels in CSF of ICH patients, and for 5-HTPи 5-HIAA there is not the corresponding increase in blood plasma. Acceleration of serotonin synthesis and degradation in brain occurs in ICH. Ratio of Trp/5-HT levels in CSF of ICH patients was more than 8 times higher versus control group. Thisindexmaybe informative for differential diagnosis between ICH and ischemic stroke.