IN VITRO CYTOTOXICITY OF CHROMATOGRAPHIC ELUTES OF CALOTROPIS PROCERA (AIT.) R BR. LEAVES AGAINST HUMAN HEPATOMA CELL LINE (HEPG2)

Calotropis procera (Ait.) R. Br. leaves extract was prepared by maceration with using a ethanol as a solvent for seven days. The dried residue was dissolved in water and partitioned with n-butanol to obtained water and n-butanol fraction. The n-butanol fraction collected was subjected to column chromatography. Chloroform, chloroform:methanol (9:1), chloroform:methanol (7:1), chloroform:methanol (1:1) and methanol elutes of n-butanol fraction of ethanolic extract of Calotropis procera (Ait) R.Br. leaves were tested against human hepatoma cell line (HEPG2) by using SRB assay method of in vitro anticancer activity.