ESTIMATION OFACECLOFENAC IN ALBINO RAT PLASMA BY USING RP-HPLC

A new RP-hPLc method for the quantitative determination of aceclofenac in albino rat plasma was developed and validated as per US-FDA guidelines. The drug was spiked in the plasma and extracted with mobile phase by precipitation method. The extracted analyte was injected into XTerra c18 (4.6 x 250 mm; 5 µm) or equivalent, maintained at 25°C temperature and effluent was monitored at 274 nm. The mobile phase consisted of potassium dihydrogen phosphate [ph 4.5]: methanol: acetonitrile [hPLc grade] (55:25:20 (V/V). The flow rate was maintained at 1.0 mL/min. The developed method showed high specificity for aceclofenac. The calibration curve for aceclofenac was linear from 25.0 to 125.0 ng/0.5mL plasma(r2= 0.999). The inter-day and intra-day precision was found to be within limits. The average % recovery for aceclofenac was found to be 99.74-99.77 % and the reproducibility was found to be satisfactory.The proposed method was adequate, sensitivity, reproducibility, and specificity for the determination ofaceclofenac in albino ratplasma.