DISSOLUTION LINKED IN VITRO LIPASE INHIBITION BY DEVELOPED ORLISTAT FORMULATION

The main objective of the study was to evaluate the lipase inhibition potential of developed formulation. Dissolution linked lipase inhibition was studied for the developed self-emulsifying tablet formulation and conventional tablet. To study lipase inhibition, pancreatic porcine lipase was selected as model enzyme for the study. A simple, rapid and cost effective colorimetric method was developed to determine the inhibition efficiency. In this method, phenyl acetate was used as the substrate for the enzymatic hydrolysis. Substrate solution was prepared in 0.1M Tris HCl buffer (pH 7) and incubated at 40°C for 20 minutes. Linearity of the method was observed in the range of 20-100μL of substrate concentration.