ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF ALISKIREN AND VALSARTAN IN BULK AND TABLET DOSAGE FORM BY RP-HPLC

A simple, precise and accurate RP-HPLC method was developed and validated for the simultaneous estimation of aliskiren and valsartan in combined tablet dosage form. The chromatographic separation of the two drugs was achieved on Symmetry C8 column (250 x 4.6 mm, 5 μm). The mobile phase consisted of acetate buffer: acetonitrile (60:40 V/V) and pH was adjusted to 5.8 with glacial acetic acid was delivered at the flow rate of 1.4 mL/min and detection was performed at 220 nm. Separation was completed within 4 minutes. The calibration curves were linear with a correlation coefficient of 0.999 over the concentration range of 7.5 to 75 µg/mL of aliskiren and 8 to 80 µg/mL of valsartan. The relative standard deviation (RSD) was found