RELATIVE POTENCY OF ANTIDIABETIC EFFECT OF COSTUS SPECIOSUS (KOEN. EX RETZ.) SMITH PLANT EXTRACT IN MOUSE FIBROBLAST CELL LINE

Plant-derived compounds have been used clinically to treat type 2 diabetes for many years as they also exert additional beneficial effects on various other disorders. PI3K pathway is the major pathway activated by insulin receptor (IR). It induces glucose uptake, glycogen synthesis, protein synthesis, cell growth and differentiation. Hence metabolic assay was employed to assess glucose uptake based on the property of 3T3-L1 cells to differentiate into adipocytes which can take up the glucose in medium due to the effect of insulin or insulin like molecules. The results of the current research showed that plants extract probably exerts its anti-diabetic properties by stimulating glucose uptake in adipocytes with significant inhibition of adipogenesis demonstrating reliable relative potency in comparision to the commercial insulin. In this preliminary in-vitro study we report Costus speciosus plant extract to have significant relative potency in reference to commercial Insulin which can be exploited to treat type II diabetes. This plant source could to be a potential, sustainable, natural anti diabetic product after structured preclinical and clinical studies. Current study leads researchers to elucidate the chemical structure, isolate active ingrediatents in the crude extract for such biological activities.