CHARACTERISTICS OF GLUTATHIONE SYSTEM IN BONE TISSUE IN LONG-TERM ENTRY OF ELEMENTS OF COPPER-ZINC PYRITIC ORES G

Earlier research showed that in mine workers who mine for copper-zinc pyritic ore by the underground method, reduction in the bone strength with development of osteopenia and osteoporosis is noted at young working age. Modelling of chronic entry of the ore components results in accumulation in experimental animals of a number of heavy metals in bone tissue with enhancement of resorption processes. The aim of research is characterization of glutathione metabolism in bone tissue in modelling of chronic entry into an organism of elements present in copper-zinc pyritic ore. Experiments were conducted on mature white male rats with daily intragastric introduction of suspension of fine powder of copper-zinc pyritic ore in starch solution in the dose calculated on the basis 60 mg/100 g of body mass with in three months. Results of the experiments showed that long-term entry into an organism of components of copper-zinc pyritic ore leads to frustration of glutathione system which plays the key role in redox status and in antioxidant protection of cells in bone tissue. In animals of the experimental group decrease in the levels of reduced glutathione, of free sulfhydryl groups, in the activity of glutathione peroxidase, glutathione-S-transferase, γ-glutamyltranspeptidase was observed. With preservation of the activity of glutathione reductase at the level of control group, in experimental animals decrease in the activity of glucose-6dehydrogenase was observed indicating disorders in production of reduced forms of NADPH in bone tissue for glutathione reduction. Thus, long-term entry of components of copper-zinc pyritic ore into an organism leads to evident disorders in glutathione system in bone tissue: decreased levels of reduced glutathione, of free thiol groups, decreased activity of glutathione-dependent enzymes and components for reduction of oxidized thiol groupings. Depletion of physiological reserves of antioxidant protection produces a negative effect on bone tissue metabolism and is one of pathochemical mechanisms of decrease in bone strength in case of prolonged contact with elements present in nonferrous metal ores.